Supporting data for “SIRT1 and stress signaling in adipose tissue”

Mice were fed with a high fat diet (HFD) for 12-weeks, starting from the age of five-weeks. Body weight and fat mass, circulating glucose, lipid and insulin levels, systemic insulin sensitivity and energy expenditure were monitored on a regular basis. At the end of treatment, serum samples were collected for analyzing the lipid contents, fatty acid composition and lipoprotein particles. Liver and adipose tissues were subjected biochemical and molecular analyses. Mitochondria were isolated from adipose tissue for structural and functional comparisons.